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A synthetic peptide corresponding to residues in C terminus of human RSK1 p90.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Our Abpromise guarantee covers the use of ab32526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: RSK1 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF-7 cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32526 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32526 was shown to recognize RSK1 when RSK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and RSK1 knockout samples were subjected to SDS-PAGE. ab32526 and ab8245 (loading control to GADPH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
ab32526 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"