使用敲除细胞株进行验证RabMAb

Anti-RSK1 p90抗体[Y81] (ab32526)

概述

  • 产品名称Anti-RSK1 p90抗体[Y81]
    参阅全部 RSK1 p90 一抗
  • 描述
    兔单克隆抗体[Y81] to RSK1 p90
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in C terminus of human RSK1 p90.

  • 阳性对照
    • MCF7 cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab32526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
IP 1/100.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IHC.
  • 靶标

    • 功能Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
    • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • 翻译后修饰Autophosphorylated on Ser-380, as part of the activation process.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 90 kDa ribosomal protein S6 kinase 1 antibody
      • dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
      • dJ590P13.1 antibody
      • EC 2.7.11.1 antibody
      • HU 1 antibody
      • HU1 antibody
      • KS6A1_HUMAN antibody
      • MAP kinase activated protein kinase 1a antibody
      • MAP kinase-activated protein kinase 1a antibody
      • MAPK-activated protein kinase 1a antibody
      • MAPKAP kinase 1a antibody
      • MAPKAPK-1a antibody
      • MAPKAPK1A antibody
      • MGC79981 antibody
      • Mitogen-activated protein kinase-activated protein kinase 1A antibody
      • OTTHUMP00000004113 antibody
      • p90 RSK1 antibody
      • p90-RSK 1 antibody
      • p90rsk antibody
      • p90RSK1 antibody
      • p90S6K antibody
      • pp90RSK1 antibody
      • Ribosomal protein S6 kinase 90kD 1 antibody
      • Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
      • Ribosomal protein S6 kinase alpha 1 antibody
      • Ribosomal protein S6 kinase alpha-1 antibody
      • Ribosomal protein S6 kinase polypeptide 1 antibody
      • Ribosomal S6 kinase 1 antibody
      • RPS6K1 alpha antibody
      • rps6ka antibody
      • Rps6ka1 antibody
      • RSK 1 antibody
      • RSK 1 p90 antibody
      • RSK antibody
      • RSK-1 antibody
      • RSK1 antibody
      • S6K alpha 1 antibody
      • S6K-alpha-1 antibody
      see all

    Anti-RSK1 p90 antibody [Y81] 图像



    • Predicted band size : 83 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: RSK1 knockout HAP1 cell lysate (20 µg)
      Lane 3: MCF-7 cell lysate (20 µg)
      Lane 4: K562 cell lysate (20 µg) 
      Lanes 1 - 4: Merged signal (red and green). Green - ab32526 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab32526 was shown to recognize RSK1 when RSK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and RSK1 knockout samples were subjected to SDS-PAGE. ab32526 and ab8245 (loading control to GADPH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Anti-RSK1 p90 antibody [Y81] (ab32526) at 1/1000 dilution + MCF7 cell lysate

      Predicted band size : 83 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)

    Anti-RSK1 p90 antibody [Y81] (ab32526)参考文献

    ab32526 has not yet been referenced specifically in any publications.

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