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A synthetic peptide corresponding to residues in C terminus of human RSK1 p90.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab32526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: RSK1 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF-7 cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32526 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32526 was shown to recognize RSK1 when RSK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and RSK1 knockout samples were subjected to SDS-PAGE. ab32526 and ab8245 (loading control to GADPH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"