重组Anti-Rsk 2 / MAPKAP Kinase 1b抗体[Y83] (ab32133)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y83] to Rsk 2 / MAPKAP Kinase 1b
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Rsk 2 / MAPKAP Kinase 1b抗体[Y83]
参阅全部 Rsk 2 / MAPKAP Kinase 1b 一抗 -
描述
兔单克隆抗体[Y83] to Rsk 2 / MAPKAP Kinase 1b -
宿主
Rabbit -
特异性
The antibody recognises Rsk2. It does not cross react with other S6 kinase family members. -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa whole cell lysate (ab150035) and human lymphoma
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y83 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32133于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
组织特异性
Expressed in many tissues, highest levels in skeletal muscle. -
疾病相关
Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻译后修饰
Autophosphorylated on Ser-386, as part of the activation process.
Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369. - Information by UniProt
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数据库链接
- Entrez Gene: 6197 Human
- Entrez Gene: 110651 Mouse
- Entrez Gene: 501560 Rat
- Omim: 300075 Human
- SwissProt: P51812 Human
- SwissProt: P18654 Mouse
- Unigene: 445387 Human
- Unigene: 328476 Mouse
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别名
- 90 kDa ribosomal protein S6 kinase 3 antibody
- CLS antibody
- HU 3 antibody
see all
图片
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Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: Rsk 2 / MAPKAP Kinase 1b knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (40 µg)
Lane 4: (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32133 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.ab32133 was shown to specifically react with Rsk 2 / MAPKAP Kinase 1b when Rsk 2 / MAPKAP Kinase 1b knockout samples were used. Wild-type and Rsk 2 / MAPKAP Kinase 1b knockout samples were subjected to SDS-PAGE. Ab32133 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773
andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776
secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging. -
ab32133, staining human lymphoma by immunohistochemistry, Paraffin embedded tissue
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133) at 1/5000 dilution + HeLa cell lysate
Predicted band size: 84 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab32133 被引用在 3 文献中.
- Xian F et al. Overexpression of phosphoprotein enriched in astrocytes 15 reverses the damage induced by propofol in hippocampal neurons. Mol Med Rep 20:1583-1592 (2019). PubMed: 31257496
- Takada I DGCR14 induces Il17a gene expression through the ROR?/BAZ1B/RSKS2 complex. Mol Cell Biol 35:344-55 (2015). WB, ChIP ; Mouse . PubMed: 25368387
- Yu G et al. RSK promotes prostate cancer progression in bone through ING3, CKAP2, and PTK6-mediated cell survival. Mol Cancer Res 13:348-57 (2015). PubMed: 25189355