概述

  • 产品名称Anti-RSF1抗体
    参阅全部 RSF1 一抗
  • 描述
    兔多克隆抗体to RSF1
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 1116-1165 (QDEFVVSDEN PDESEEDPPS NDDSDTDFCS RRLRRHPSRP MRQSRRLRRK) of human RSF1 (NP_057662).

  • 阳性对照
    • Human fetal lung lysate

性能

应用

Our Abpromise guarantee covers the use of ab83322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:12500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 163 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性RSF1 is involved in transcription repression when recruited to DNA via the DNA binding of GAL4. It is also involved in chromatin remodeling and spacing when associated with SNF2H and transcription coactivation when associated with hepatitis B virus X protein (HBX). The RSF1 gene is localized to the long arm of human chromosome 11 between q13.4 and q14.1. This region is amplified and rearranged in many tumors, indicating that it plays a role in tumorigenesis similar to that of other PHD-containing proteins.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • HBV pX associated protein 8 antibody
    • Hepatitis B virus x associated protein antibody
    • HXBAP antibody
    • p325 antibody
    • p325 subunit of RSF chromatin remodeling complex antibody
    • remodeling and spacing factor 1 antibody
    • RSF 1 antibody
    • XAP8 antibody
    see all

Anti-RSF1 antibody 图像

  • Anti-RSF1 antibody (ab83322) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 163 kDa
    Observed band size : 168 kDa (why is the actual band size different from the predicted?)

Anti-RSF1 antibody (ab83322)参考文献

ab83322 has not yet been referenced specifically in any publications.

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