antigen sequence, corresponding to C terminal amino acids 702-828 of Human RSBN1L.
Human cerebellum tissue; RT4, Human Plasma and Tonsil lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/250 - 1/500.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-RSBN1L antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-RSBN1L antibody (ab121626)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and plasma membrane. Recommended concentration of ab121626 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSBN1L antibody (ab121626)
ab121626 at 1/65 dilution staining RSBN1L in Paraffin-embedded Human Cerebellum tissue by Immunohistochemistry.
Western blot - Anti-RSBN1L antibody (ab121626)
All lanes : Anti-RSBN1L antibody (ab121626) at 1/250 dilution Lane 1 : RT 4 lysate Lane 2 : U 251 MG lysate Lane 3 : Human Plasma lysate Lane 4 : Human Liver lysate Lane 5 : Human Tonsil lysate
Anti-RSBN1L antibody (ab121626)参考文献
has not yet been referenced specifically in any publications.
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