概述

  • 产品名称(RS)-CHPG
  • 描述
    Selective mGlu5 agonist
  • 生物学描述Selective mGlu5 receptor agonist. Water soluble form available - see (ab120221)
  • 纯度> 98%

性能

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    应用

    Our Abpromise guarantee covers the use of ab120039 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    (R,S)-CHPG 图像

    • SK-N-SH cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and different concentrations of (R,S)-CHPG (ab120039). Increased expression of CREB (phospho S133) in SK-N-SH cells correlates with an increase in (R,S)-CHPG concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab32096 at 1/500 dilution and ab32515 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    (R,S)-CHPG (ab120039)参考文献

    This product has been referenced in:
    • Domenici MR  et al. Chronic treatment with the mGlu5R antagonist MPEP reduces the functional effects of the mGlu5R agonist CHPG in the striatum of 6-hydroxydopamine-lesioned rats: possible relevance to the effects of mGlu5R blockade in Parkinson's disease. J Neurosci Res 80:646-54 (2005). Read more (PubMed: 15880742) »
    • Salt TE  et al. Antagonism of the mGlu5 agonist 2-chloro-5-hydroxyphenylglycine by the novel selective mGlu5 antagonist 6-methyl-2-(phenylethynyl)-pyridine (MPEP) in the thalamus. Br J Pharmacol 127:1057-9 (1999). Read more (PubMed: 10455248) »
    • Doherty AJ  et al. (RS)-2-chloro-5-hydroxyphenylglycine (CHPG) activates mGlu5, but no mGlu1, receptors expressed in CHO cells and potentiates NMDA responses in the hippocampus. Neuropharmacology 36:265-7 (1997). Read more (PubMed: 9144665) »

    See all 3 Publications for this product

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