Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to a region within internal sequence amino acids 684-733 (VTFGLNRNMT AEFKKTDKSI LVSPTGPSRV AFDPEQKPLH GVLKTPTSSP) of Human RRP1B (NP_055871).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Anti-RRP1B antibody 图像
Western blot - RRP1B antibody (ab84522)
Anti-RRP1B antibody (ab84522) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
84 kDaObserved band size :
90 kDa (why is the actual band size different from the predicted?
)Additional bands at :
35 kDa. We are unsure as to the identity of these extra bands.
Anti-RRP1B antibody (ab84522)参考文献
has not yet been referenced specifically in any publications.
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