概述

  • 产品名称Anti-RRN3抗体
    参阅全部 RRN3 一抗
  • 描述
    兔多克隆抗体to RRN3
  • 特异性ab79375 detects endogenous levels of total RRN3 protein.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human RRN3 around the phosphorylation site of serine 649 (Q-P-SP-P-L).

  • 阳性对照
    • HeLa cell extracts, treated with calyculin A (50ng/ml, 30mins).

性能

应用

Our Abpromise guarantee covers the use of ab79375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).

靶标

  • 功能Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.
  • 序列相似性Belongs to the RRN3 family.
  • 翻译后修饰Phosphorylation is required for participation in rDNA transcription.
  • 细胞定位Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AL023001 antibody
    • DKFZp566E104 antibody
    • E130302O19Rik antibody
    • MGC104238 antibody
    • R75565 antibody
    • RGD1305001 antibody
    • RNA polymerase I-specific transcription initiation factor RRN3 antibody
    • Rrn3 antibody
    • RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae) antibody
    • RRN3_HUMAN antibody
    • TIF-IA antibody
    • TIF1A antibody
    • TIFIA antibody
    • Transcription Initiation Factor 1A antibody
    • Transcription initiation factor IA antibody
    • Transcription initiation factor TIF IA antibody
    see all

Anti-RRN3 antibody 图像

  • All lanes : Anti-RRN3 antibody (ab79375) at 1/500 dilution

    Lane 1 : HeLa cell extracts, treated with calyculin A (50ng/ml, 30mins)
    Lane 2 : HeLa cell extracts, treated with calyculin A (50ng/ml, 30mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 74 kDa
    Observed band size : 74 kDa

Anti-RRN3 antibody (ab79375)参考文献

ab79375 has not yet been referenced specifically in any publications.

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