概述

  • 产品名称
  • 描述
    兔多克隆抗体to RRN3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Dog
  • 免疫原

    A region within synthetic peptide: KKDIVEDEDD DFLKGEVPQN DTVIGITPSS FDTHFRSPSS SVGSPPVLYM, corresponding to amino acids 597-646 of Human RRN3

  • 阳性对照
    • Jurkat cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab42539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 74 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.
  • 序列相似性
    Belongs to the RRN3 family.
  • 翻译后修饰
    Phosphorylation is required for participation in rDNA transcription.
  • 细胞定位
    Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AL023001 antibody
    • DKFZp566E104 antibody
    • E130302O19Rik antibody
    • MGC104238 antibody
    • R75565 antibody
    • RGD1305001 antibody
    • RNA polymerase I-specific transcription initiation factor RRN3 antibody
    • Rrn3 antibody
    • RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae) antibody
    • RRN3_HUMAN antibody
    • TIF-IA antibody
    • TIF1A antibody
    • TIFIA antibody
    • Transcription Initiation Factor 1A antibody
    • Transcription initiation factor IA antibody
    • Transcription initiation factor TIF IA antibody
    see all

图片

  • Anti-RRN3 antibody (ab42539) at 0.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 74 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab42539 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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