Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.
Belongs to the RRN3 family.
Phosphorylation is required for participation in rDNA transcription.
RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae) antibody
Transcription Initiation Factor 1A antibody
Transcription initiation factor IA antibody
Transcription initiation factor TIF IA antibody
Western blot - RRN3 antibody (ab112052)
All lanes : Anti-RRN3 antibody (ab112052) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
Exposure time: 3 minutes
Immunoprecipitation - RRN3 antibody (ab112052)
ab112052 at 1 µg/ml staining RRN3 in HeLa whole cell lysate immunoprecipitated using ab112052 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: controle IgG.
Predicted band size : 74 kDa
Exposure time: 10 seconds
Ueshima S et al. Upstream binding factor-dependent and pre-rRNA transcription-independent association of pre-rRNA processing factors with rRNA gene. Biochem Biophys Res Commun443:22-7 (2014).
Read more (PubMed: 24269811) »