概述

  • 产品名称
  • 描述
    兔多克隆抗体to RRN3
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 601-651 of Human RRN3 (NP_060897.3 (GeneID 54700)).

  • 阳性对照
    • HeLa and Jurkat whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab112052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 74 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.
  • 序列相似性
    Belongs to the RRN3 family.
  • 翻译后修饰
    Phosphorylation is required for participation in rDNA transcription.
  • 细胞定位
    Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AL023001 antibody
    • DKFZp566E104 antibody
    • E130302O19Rik antibody
    • MGC104238 antibody
    • R75565 antibody
    • RGD1305001 antibody
    • RNA polymerase I-specific transcription initiation factor RRN3 antibody
    • Rrn3 antibody
    • RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae) antibody
    • RRN3_HUMAN antibody
    • TIF-IA antibody
    • TIF1A antibody
    • TIFIA antibody
    • Transcription Initiation Factor 1A antibody
    • Transcription initiation factor IA antibody
    • Transcription initiation factor TIF IA antibody
    see all

图片

  • All lanes : Anti-RRN3 antibody (ab112052) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 74 kDa


    Exposure time : 3 minutes
  • ab112052 at 1 µg/ml staining RRN3 in HeLa whole cell lysate immunoprecipitated using ab112052 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: controle IgG. Predicted band size : 74 kDa Exposure time: 10 seconds

文献

This product has been referenced in:
  • Ueshima S  et al. Upstream binding factor-dependent and pre-rRNA transcription-independent association of pre-rRNA processing factors with rRNA gene. Biochem Biophys Res Commun 443:22-7 (2014). Read more (PubMed: 24269811) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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