- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-RRN3抗体
参阅全部 RRN3 一抗 -
描述兔多克隆抗体to RRN3
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宿主Rabbit
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经测试应用适用于: WB, IPmore details
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种属反应性与反应: Human
预测可用于: Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide, corresponding to a region between amino acids 601-651 of Human RRN3 (NP_060897.3 (GeneID 54700)).
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阳性对照
- HeLa and Jurkat whole cell lysate
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液pH: 7.50
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab112052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
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| WB | 1/2000 - 1/10000. Predicted molecular weight: 74 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
靶标
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功能Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.
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序列相似性Belongs to the RRN3 family.
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翻译后修饰Phosphorylation is required for participation in rDNA transcription.
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细胞定位Nucleus > nucleolus.
- Information by UniProt
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数据库链接
- Entrez Gene: 54700 Human
- Entrez Gene: 100173945 Orangutan
- Omim: 605121 Human
- SwissProt: Q9NYV6 Human
- SwissProt: Q5R4N9 Orangutan
- Unigene: 460078 Human
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别名
- AL023001 antibody
- DKFZp566E104 antibody
- E130302O19Rik antibody
see all
图片
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Western blot - RRN3 antibody (ab112052)All lanes : Anti-RRN3 antibody (ab112052) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
Exposure time: 3 minutes -
Immunoprecipitation - RRN3 antibody (ab112052)ab112052 at 1 µg/ml staining RRN3 in HeLa whole cell lysate immunoprecipitated using ab112052 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: controle IgG. Predicted band size : 74 kDa Exposure time: 10 seconds
文献
This product has been referenced in:
- Ueshima S et al. Upstream binding factor-dependent and pre-rRNA transcription-independent association of pre-rRNA processing factors with rRNA gene. Biochem Biophys Res Commun 443:22-7 (2014). Read more (PubMed: 24269811) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"