重组Anti-RRM1抗体[EPR8483] (ab137114)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8483] to RRM1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-RRM1抗体[EPR8483]
参阅全部 RRM1 一抗 -
描述
兔单克隆抗体[EPR8483] to RRM1 -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
-
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human RRM1 aa 700-800. The exact sequence is proprietary.
Database link: P23921 -
阳性对照
- WB: HT-29, A549, MCF7 and HeLa whole cell lysate (ab150035), Mouse colon, Rat brain, and Rat colon lysates. IHC-P: human lung and colon carcinoma tissues. ICC/IF: HT-29 cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8483 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137114于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100.
|
|
WB |
1/10000 - 1/50000. Predicted molecular weight: 90 kDa.
|
|
ICC/IF | (1) |
1/100 - 1/250.
|
IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
说明 |
---|
Flow Cyt (Intra)
1/100. |
WB
1/10000 - 1/50000. Predicted molecular weight: 90 kDa. |
ICC/IF
1/100 - 1/250. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
靶标
-
功能
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. -
通路
Genetic information processing; DNA replication. -
序列相似性
Belongs to the ribonucleoside diphosphate reductase large chain family.
Contains 1 ATP-cone domain. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 6240 Human
- Entrez Gene: 20133 Mouse
- Entrez Gene: 685579 Rat
- Omim: 180410 Human
- SwissProt: P23921 Human
- SwissProt: P07742 Mouse
- Unigene: 445705 Human
- Unigene: 197486 Mouse
-
别名
- R1 antibody
- Ribonucleoside diphosphate reductase large subunit antibody
- Ribonucleoside diphosphate reductase M1 chain antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling RRM1 with purified ab137114 at 1/1000 dilution (0.95 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
All lanes : Anti-RRM1 antibody [EPR8483] (ab137114) at 1/10000 dilution (Purified)
Lane 1 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 3 : Mouse colon lysates
Lane 4 : Rat brain lysates
Lane 5 : Rat colon lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa -
Immunocytochemistry/ Immunofluorescence analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling RRM1 with purified ab137114 at 1/100 dilution (9.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling RRM1 with ab137114 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
unpurified ab137114 staining RRM1 in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
-
All lanes : Anti-RRM1 antibody [EPR8483] (ab137114) at 1/10000 dilution ((unpurified))
Lane 1 : HT29 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP-labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 90 kDa -
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling RRM1 with unpurified ab137114 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Unpurified ab137114 (1/200) staining RRM1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5%Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (15)
ab137114 被引用在 15 文献中.
- Ueno H et al. TAS1553, a small molecule subunit interaction inhibitor of ribonucleotide reductase, exhibits antitumor activity by causing DNA replication stress. Commun Biol 5:571 (2022). PubMed: 35681099
- Liu Z et al. Association between TOP2A, RRM1, HER2, ERCC1 expression and response to chemotherapy in patients with non-muscle invasive bladder cancer. Heliyon 8:e09643 (2022). PubMed: 35711974
- Maruyama A et al. De novo deoxyribonucleotide biosynthesis regulates cell growth and tumor progression in small-cell lung carcinoma. Sci Rep 11:13474 (2021). PubMed: 34188151
- Zhou J et al. Identification of chemoresistance-related mRNAs based on gemcitabine-resistant pancreatic cancer cell lines. Cancer Med 9:1115-1130 (2020). PubMed: 31823522
- Okonska A et al. Functional Genomic Screen in Mesothelioma Reveals that Loss of Function of BRCA1-Associated Protein 1 Induces Chemoresistance to Ribonucleotide Reductase Inhibition. Mol Cancer Ther 19:552-563 (2020). PubMed: 31619462