概述

  • 产品名称Anti-RRM1抗体
    参阅全部 RRM1 一抗
  • 描述
    兔多克隆抗体to RRM1
  • 经测试应用适用于: WB, ICC, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from the C-terminus of human RRM1.

  • 阳性对照
    • Human placenta.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab81085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
ICC Use at an assay dependent concentration. PubMed: 23840376
IP Use at an assay dependent concentration.
IHC-P 1/100.

靶标

  • 功能Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
  • 通路Genetic information processing; DNA replication.
  • 序列相似性Belongs to the ribonucleoside diphosphate reductase large chain family.
    Contains 1 ATP-cone domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • R1 antibody
    • Ribonucleoside diphosphate reductase large subunit antibody
    • Ribonucleoside diphosphate reductase M1 chain antibody
    • Ribonucleoside diphosphate reductase subunit M1 antibody
    • Ribonucleoside reductase, large subunit antibody
    • Ribonucleoside-diphosphate reductase large subunit antibody
    • Ribonucleoside-diphosphate reductase subunit M1 antibody
    • Ribonucleotide reductase large chain antibody
    • Ribonucleotide reductase large subunit antibody
    • Ribonucleotide reductase M1 antibody
    • Ribonucleotide reductase M1 polypeptide antibody
    • Ribonucleotide reductase R1 subunit antibody
    • Ribonucleotide reductase, M1 subunit antibody
    • RIR 1 antibody
    • RIR1 antibody
    • RIR1_HUMAN antibody
    • RR 1 antibody
    • RR1 antibody
    • RRM 1 antibody
    • RRM1 antibody
    see all

Anti-RRM1 antibody 图像

  • ab81085, at 1/100 dilution, staining RRM1 in Human Placenta by Immunohistochemistry using formalin-fixed paraffin-embedded tissue.
  • Anti-RRM1 antibody (ab81085) at 1/500 dilution + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 90 kDa
    Observed band size : 90 kDa
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • RRM1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to RRM1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab81085.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697)..
    Band: Band: 90kDa: RRM1.
  • Immunohistochemical analysis of Human pancreatic ductal adenocarcinoma tissue, staining RRM1 with ab81085 at 1/50 dilution.

Anti-RRM1 antibody (ab81085)参考文献

This product has been referenced in:
  • Szulkin A  et al. Variation in drug sensitivity of malignant mesothelioma cell lines with substantial effects of selenite and bortezomib, highlights need for individualized therapy. PLoS One 8:e65903 (2013). ICC ; Human . Read more (PubMed: 23840376) »
  • Valsecchi ME  et al. Is there a role for the quantification of RRM1 and ERCC1 expression in pancreatic ductal adenocarcinoma? BMC Cancer 12:104 (2012). IHC-P ; Human . Read more (PubMed: 22436573) »

See all 4 Publications for this product

Product Wall

Thank you for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge vial of ab81085 on the order ***, which should arrive on Tuesday.

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Thank you very much for your interest in our RRM1 antibodies.

While we have not tested any of our antibodies for use on mouse samples, the mouse and human RRM1 proteins are 98% homologous. Therefore, I can offer a discount off a future purch...

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Thank you for your call today and for your patience while I've been in touch with the lab about this antibody.

The concentration of the lot you're using is 0.2 mg/mL.

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Thank you for your telephone enquiry yesterday.

I can confirm that the concentration of is lot specific. The concentration of lotGR-76187-1 is 0.2 mg/ml.

I hope this will be helpful to you. If you have any further questions, pleas...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"