概述

  • 产品名称Anti-RRAS2抗体
    参阅全部 RRAS2 一抗
  • 描述
    小鼠单克隆抗体to RRAS2
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-205 of Human RRAS2

性能

应用

Our Abpromise guarantee covers the use of ab56859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能It is a plasma membrane-associated GTP-binding protein with GTPase activity. Might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins but in an antagonistic fashion.
  • 组织特异性Ubiquitously present in all tissues examined, with the highest levels in heart, placenta, and skeletal muscle. Moderate levels in lung and liver; low levels in brain, kidney, and pancreas.
  • 疾病相关Ovarian cancer
  • 序列相似性Belongs to the small GTPase superfamily. Ras family.
  • 翻译后修饰May be post-translationally modified by both palmitoylation and polyisoprenylation.
  • 细胞定位Cell membrane. Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine (By similarity with RAS).
  • Information by UniProt
  • 数据库链接
  • 别名
    • C86394 antibody
    • Oncogene RRAS2 antibody
    • Oncogene TC21 antibody
    • Ras like protein TC21 antibody
    • Ras related protein R Ras2 antibody
    • Ras-like protein TC21 antibody
    • Ras-related protein R-Ras2 antibody
    • Related RAS viral (r ras) oncogene homolog 2 antibody
    • Related RAS viral oncogene homolog 2 antibody
    • RRAS 2 antibody
    • RRAS2 antibody
    • RRAS2_HUMAN antibody
    • TC 21 antibody
    • TC21 antibody
    • Teratocarcinoma oncogene antibody
    • Teratocarcinoma oncogene TC21 antibody
    see all

Anti-RRAS2 antibody 图像

  • Overlay histogram showing A431 cells stained with ab56859 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56859, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • RRAS2 antibody (ab56859) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human dysgerminoma tissue.
  • RRAS2 antibody (ab56859) at 1ug/lane + A-431 cell lysate at 25ug/lane.

Anti-RRAS2 antibody (ab56859)参考文献

This product has been referenced in:
  • Dahiya N  et al. Claudin-7 is frequently overexpressed in ovarian cancer and promotes invasion. PLoS One 6:e22119 (2011). WB ; Human . Read more (PubMed: 21789222) »

See 1 Publication for this product

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