Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RRAS.
(Peptide available as ab93071.)
- This antibody gave a positive signal in human skeletal muscle as well as the following whole cell lysates: HeLa; HT29; DU145; PC3; A549.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/250. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Regulates the organization of the actin cytoskeleton.
Belongs to the small GTPase superfamily. Ras family.
Cell membrane. Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine.
Information by UniProt
- Oncogene RRAS antibody
- p23 antibody
- R Ras antibody
Western blot - RRAS antibody (ab82356)
All lanes :
Anti-RRAS antibody (ab82356) at 1/250 dilutionLane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 :
HT29 (Human colon adenocarcinoma) Whole Cell LysateLane 3 :
Human skeletal muscle tissue lysate - total protein (ab29330
)Lane 4 :
DU 145 (Human prostate carcinoma cell line) Whole Cell LysateLane 5 : PC3 whole cell lysate (ab3954)
Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 24 kDa (why is the actual band size different from the predicted?)
Additional bands at: 40 kDa, 63 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"