概述

  • 产品名称Anti-RRAGB抗体
    参阅全部 RRAGB 一抗
  • 描述
    小鼠单克隆抗体to RRAGB
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-347 of Human RRAGB

性能

应用

Our Abpromise guarantee covers the use of ab56633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway.
    • 序列相似性Belongs to the GTR/RAG GTP-binding protein family.
    • 细胞定位Cytoplasm. Lysosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • GTP-binding protein ragB antibody
      • Rag B antibody
      • RagB antibody
      • Ras-related GTP-binding protein B antibody
      • RRAGB antibody
      • RRAGB_HUMAN antibody
      see all

    Anti-RRAGB antibody 图像

    • Western blot against tagged recombinant protein immunogen using ab56633 RRAGB antibody at 1ug/ml. Predicted band size of immunogen is 64 kDa

    Anti-RRAGB antibody (ab56633)参考文献

    This product has been referenced in:
    • Sancak Y  et al. The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Science 320:1496-501 (2008). WB ; Human . Read more (PubMed: 18497260) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa, 293T)
    Loading amount 15 µg
    Specification HeLa, 293T
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    提交于 Nov 18 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"