概述

  • 产品名称Anti-RRAD抗体
    参阅全部 RRAD 一抗
  • 描述
    兔多克隆抗体to RRAD
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal region of human RRAD.

  • 阳性对照
    • Human heart tissue. Extracts from HeLa cells, HepG2 cells and MCF-7 cells. A549 cells.

性能

应用

Our Abpromise guarantee covers the use of ab75100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.
  • 组织特异性Most abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans.
  • 序列相似性Belongs to the small GTPase superfamily. RGK family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GTP binding protein RAD antibody
    • GTP-binding protein RAD antibody
    • RAD antibody
    • RAD_HUMAN antibody
    • RAD1 antibody
    • RAS (RAD and GEM) like GTP binding 3 antibody
    • Ras associated with diabetes antibody
    • Ras related associated with diabetes antibody
    • REM3 antibody
    • RRAD antibody
    see all

Anti-RRAD antibody 图像

  • All lanes : Anti-RRAD antibody (ab75100) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from HepG2 cells
    Lane 3 : Extracts from MCF-7 cells
    Lane 4 : Extracts from HeLa cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of RRAD in paraffin-embedded human heart tissue using ab75100, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • Immunofluorescence analysis of RRAD in A549 cells using ab75100, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-RRAD antibody (ab75100)参考文献

ab75100 has not yet been referenced specifically in any publications.

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