概述

  • 产品名称Anti-Rpt3抗体
  • 描述
    兔多克隆抗体to Rpt3
  • 特异性Reacts with yeast proteasomes.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae
  • 免疫原

    His6 tagged recombinant fragment:

    MEELGIVTPVEKAVEEKPAVKSYASLLAQLNGTVNNNSALSNVNSDIYFK LKKLEKEYELLTLQEDYIKDEQRHLKRELKRAQEEVKRIQSVPLVIGQFL

    , corresponding to N terminal amino acids 1-100 of yeast, S. cerevisiae Rpt3 (YTA2) protein sequence.

  • 阳性对照
    • Yeast whole cell extract and purified yeast 26S proteasome.
  • 常规说明Store unopened vial at -20°C until required for use. Aliquot undiluted antibody into smaller volumes (not less than 10µL) prior to freezing if appropriate. Antibody can be diluted to working strength with PBS, pH 7.2 to 7.4, and stored at 4°C for up to 4 weeks but do not freeze.

性能

应用

Our Abpromise guarantee covers the use of ab22677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

靶标

  • 相关性In the yeast, S. cerevisiae, 12 different members of a novel gene family of putative ATPases have been identified and characterised. Due to their similarity to TBP1, a human immunodeficiency tat binding protein, they have been designated the YTA family (yeast tatbinding analogues). The most outstanding feature of the YTA proteins and their relatives is that they share a region of high similarity which extends to some 300 amino acids in length. The amino acid sequences preceding the region of high similarity are less well conserved but do contain similar structural domains, i.e. coil to coil propensities, leucine zipper like structures. However, despite the highly conserved structural features among the YTA family, diverse functions have been assigned to the various family members. The putative gene products of YTA1, YTA2, YTA3 and YTA5 are all closely related to proteins known to be constituents of the human 26S proteasome. YTA2 or Rpt3 exhibits 81% overall similarity and 62% identity at the amino acid level with human TBP7 and is regarded as the yeast homologue of human proteasomal subunit TBP7. Rpt3 has been shown to be an essential gene in yeast.
  • 细胞定位Cytoplasmic and nuclear
  • 数据库链接
    • 别名
      • 26S protease regulatory subunit 6B homolog antibody
      • Protein YNT1 antibody
      • Rpt 3 antibody
      • TBP7 antibody
      • YNT1 antibody
      • YTA2 antibody
      see all

    Anti-Rpt3 antibody 图像

    • Anti-Rpt3 antibody (ab22677) at 1/25000 dilution (detection on PVDF membrane)
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 48 kDa
      Observed band size : 48 kDa


      Exposure time : 1 minute

    Anti-Rpt3 antibody (ab22677)参考文献

    ab22677 has not yet been referenced specifically in any publications.

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