Anti-Rpt1抗体(ab22678)

概述

  • 产品名称Anti-Rpt1抗体
    参阅全部 Rpt1 一抗
  • 描述
    兔多克隆抗体to Rpt1
  • 特异性At optimal dilution there is no cross-reactivity with other members of the YTA family.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae, Schizosaccharomyces pombe
  • 免疫原

    Recombinant (His-tagged) fusion protein:

    MPPKEDWEKY KAPLEDDDKK PDDDKIVPLT EGDIQVLKSY GAAPYAAKLK QTENDLKDIE ARIKEKAGVK ESDTGLAPSH LWDIMGDRQR

    LGEEHPLQVA, corresponding to amino acids 1-100 of Rpt1 (yeast).

  • 常规说明Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).

性能

应用

Our Abpromise guarantee covers the use of ab22678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa.

靶标

  • 相关性Rpt1 in yeast is one of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and the E3 ubiquitin-protein ligase Ubr1p. In the yeast S. cerevisiae, 12 different members of a novel gene family of putative ATPases have been identified and characterised. Due to their relationship with TBP1, a human immunodeficiency tat binding protein, they have been designated the YTA family (yeast tat binding analogues). The most outstanding feature of the YTA proteins and their relatives is that they share a region of high similarity which extends to some 300 amino acids in length.
  • 细胞定位Cytoplasmic and Nuclear
  • 数据库链接
    • 别名
      • 26S protease regulatory subunit 7 homolog antibody
      • CIM5 antibody
      • Protein CIM5 antibody
      • TAT-binding homolog 3 antibody
      • YTA3 antibody
      see all

    Anti-Rpt1 antibody 图像

    • Anti-Rpt1 antibody (ab22678) at 1/25000 dilution + Purified yeast 26S proteasome preparation
      Developed using the ECL technique

      Exposure time : 1 minute

    Anti-Rpt1 antibody (ab22678)参考文献

    ab22678 has not yet been referenced specifically in any publications.

    Product Wall

    Please find below some information about the testing conditions:

    Western blot - Single dimension SDS-PAGE of a yeast whole cell extract and purified yeast 26S proteasome followed by Western blotting shows a major band at ˜52kDa. The antibody...

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    Thank you for contacting Abcam regarding ab22678 and for taking the time to provide some details of the customer's Western blot experiments. I am very sorry to hear that he/she is having problem with this particular product. Here I am forwarding my...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"