Anti-RPS6 (phospho S244 + S247)抗体(ab12865)

概述

  • 产品名称Anti-RPS6 (phospho S244 + S247)抗体
    参阅全部 RPS6 一抗
  • 描述
    兔多克隆抗体to RPS6 (phospho S244 + S247)
  • 特异性This antibody does not cross react with RPS6 phosphorylated on serines 235 and 236.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from the region of human RPS6 that contains serines 244 and 247 (unfortunately, the amino acid sequence is considered to be commercially sensitive)

  • 阳性对照
    • HeLa +/- TNF-alpha or anisomycin
  • 常规说明Centrifuge before opening to settle contents.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: Dulbecco's PBS without magnesium and calcium, 50% Glycerol, PBS, 1mg/ml BSA, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated RPS6. The final product is generated by affinity chromatography using a RPS6-derived peptide that is phosphorylated at serines 244 and 247.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab12865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).

靶标

Anti-RPS6 (phospho S244 + S247) antibody 图像



  • Predicted band size : 31 kDa


    Lysates prepared from HeLa cells left untreated (1) or treated with TNF-alpha (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab12865 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat anti-rabbit IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to RPS6 [phospho S244 & phospho S247] blocks the signal, verifying the specificity of the antibody.

Anti-RPS6 (phospho S244 + S247) antibody (ab12865)参考文献

This product has been referenced in:
  • Järås M  et al. Csnk1a1 inhibition has p53-dependent therapeutic efficacy in acute myeloid leukemia. J Exp Med 211:605-12 (2014). WB ; Human . Read more (PubMed: 24616378) »

See 1 Publication for this product

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