Anti-RPS6 (phospho S240 + S244)抗体(ab53024)

概述

  • 产品名称Anti-RPS6 (phospho S240 + S244)抗体
    参阅全部 RPS6 一抗
  • 描述
    兔多克隆抗体to RPS6 (phospho S240 + S244)
  • 特异性This antibody detects endogenous levels of S6 Ribosomal protein only when phosphorylated at serine 240 or serine 244.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from human S6 Ribosomal protein around the phosphorylation site of serine 240 or serine 244 (SP-T-S-K-SP)

  • 阳性对照
    • Extracts from 293 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 30 kDa.
ELISA 1/20000.

靶标

Anti-RPS6 (phospho S240 + S244) antibody 图像

  • All lanes : Anti-RPS6 (phospho S240 + S244) antibody (ab53024) at 1/500 dilution

    Lane 1 : Extracts from 293 cells treated with serum (10%, 15mins) with no immunizing peptide
    Lane 2 : Extracts from 293 cells treated with serum (10%, 15mins) with immunizing peptide


    Predicted band size : 30 kDa

Anti-RPS6 (phospho S240 + S244) antibody (ab53024)参考文献

ab53024 has not yet been referenced specifically in any publications.

Product Wall



Unfortunately, we have not tested this antibody in mouse samples and therefore we do not have a western blot with which to compare yours to. As for the identity of the extra band, we have never seen this band before and when I performed a lit...

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