Anti-RPS6 (phospho S235 + S236)抗体(ab12864)

概述

  • 产品名称Anti-RPS6 (phospho S235 + S236)抗体
    参阅全部 RPS6 一抗
  • 描述
    兔多克隆抗体to RPS6 (phospho S235 + S236)
  • 特异性This antibody does not cross react with RPS6 phosphorylated on serines 244 and 247.
  • 经测试应用适用于: WB, ICC/IF, Electron Microscopymore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from the region of human RPS6 that contains serines 235 and 236 (unfortunately, the amino acid sequence is considered to be commercially sensitive)

  • 阳性对照
    • HeLa +/- TNF-alpha or anisomycin
  • 常规说明Centrifuge before opening to settle contents.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: Dulbecco's PBS without magnesium and calcium, 50% Glycerol, PBS, 1mg/ml BSA, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated RPS6. The final product is generated by affinity chromatography using a RPS6-derived peptide that is phosphorylated at serines 235 and 236.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab12864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).
ICC/IF Use at an assay dependent concentration. Used at a dilution of 1/100 for 2 hrs on HeLa cells (see Abreview submitted by Marco Biggiogera).
Electron Microscopy Use at an assay dependent dilution.

靶标

Anti-RPS6 (phospho S235 + S236) antibody 图像



  • Predicted band size : 31 kDa


    Peptide Competition. Lysates prepared from HeLa cells left untreated (1) or treated with anisomycin (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab12864 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with a goat anti-rabbit IgG HRP conjugate secondary antibody and bands were detected using the Pierce SuperSignalTM method. The data show that only the peptide corresponding to RPS6 blocks the signal, verifying the specificity of the antibody.
  •  ab12864 at a 1/100 dilution staining human HeLa cells by ICC/IF. The cells were formaldehyde fixed and then incubated with the antibody for 2 hours. An Alexa Fluor ® 488 conjugated goat polyclonal antibody was used as the secondary.

    See Abreview

Anti-RPS6 (phospho S235 + S236) antibody (ab12864)参考文献

This product has been referenced in:
  • Wang M  et al. Role of ribosomal protein RPS2 in controlling let-7a expression in human prostate cancer. Mol Cancer Res 9:36-50 (2011). WB ; Human . Read more (PubMed: 21148031) »
  • Shen Y  et al. Mutations in PNKD causing paroxysmal dyskinesia alters protein cleavage and stability. Hum Mol Genet 20:2322-32 (2011). ICC/IF ; Human . Read more (PubMed: 21487022) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HeLa)
Loading amount 50 µg
Specification HeLa
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5%
Username

Dr. Marco Biggiogera

Verified customer

提交于 Sep 15 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Username

Dr. Marco Biggiogera

Verified customer

提交于 Aug 09 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"