Anti-RPS6 (phospho S235 + S236)抗体(ab12864)

概述

  • 产品名称
    Anti-RPS6 (phospho S235 + S236)抗体
    参阅全部 RPS6 一抗
  • 描述
    兔多克隆抗体to RPS6 (phospho S235 + S236)
  • 特异性
    This antibody does not cross react with RPS6 phosphorylated on serines 244 and 247.
  • 经测试应用
    适用于: WB, ICC/IF, Electron Microscopymore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from the region of human RPS6 that contains serines 235 and 236 (unfortunately, the amino acid sequence is considered to be commercially sensitive)

  • 阳性对照
    • HeLa +/- TNF-alpha or anisomycin
  • 常规说明
    Centrifuge before opening to settle contents.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Sodium Azide
    Constituents: Dulbecco's PBS without magnesium and calcium, 50% Glycerol, PBS, 1mg/ml BSA, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated RPS6. The final product is generated by affinity chromatography using a RPS6-derived peptide that is phosphorylated at serines 235 and 236.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab12864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).
ICC/IF Use at an assay dependent concentration. Used at a dilution of 1/100 for 2 hrs on HeLa cells (see Abreview submitted by Marco Biggiogera).
Electron Microscopy Use at an assay dependent dilution.

靶标

Anti-RPS6 (phospho S235 + S236) antibody 图像



  • Predicted band size : 31 kDa
    Peptide Competition.
    Lysates prepared from HeLa cells left untreated (1) or treated with anisomycin (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room  temperature, and incubated with ab12864 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with a goat anti-rabbit IgG HRP conjugate secondary antibody and bands were detected using the Pierce SuperSignalTM method. The data show that only the peptide corresponding to RPS6 blocks the signal, verifying the specificity of the antibody. Peptide Competition. Lysates prepared from HeLa cells left untreated (1) or treated with anisomycin (2-5) were resolved by SDS-PAGE on a 14% po
  •  ab12864 at a 1/100 dilution staining human HeLa cells by ICC/IF. The cells were formaldehyde fixed and then incubated with the antibody for 2 hours. An Alexa Fluor ® 488 conjugated goat polyclonal antibody was used as the secondary.

    See Abreview

Anti-RPS6 (phospho S235 + S236) antibody (ab12864)参考文献

This product has been referenced in:
  • Fan G  et al. Danshensu alleviates cardiac ischaemia/reperfusion injury by inhibiting autophagy and apoptosis via activation of mTOR signalling. J Cell Mol Med 20:1908-19 (2016). Rat . Read more (PubMed: 27385290) »
  • Van Rechem C  et al. Lysine demethylase KDM4A associates with translation machinery and regulates protein synthesis. Cancer Discov 5:255-63 (2015). Human . Read more (PubMed: 25564516) »

See all 6 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse (macrophage, skeletal muscle) Human (kidney))
Gel Running Conditions
Reduced Denaturing (8-18% gradient)
Loading amount
5 µg
Treatment
293A cells were treated with mTORC1 catalytic inhibitor 50nM for 1hr to inhibit S6 phosphorylation
Specification
Mouse (macrophage, skeletal muscle) Human (kidney)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jun 21 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Bos taurus Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (4-15% Gradient)
Loading amount
20 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Julie Kim

Verified customer

提交于 May 04 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (HeLa)
Loading amount
50 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 5%
Username

Dr. Marco Biggiogera

Verified customer

提交于 Sep 15 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Username

Dr. Marco Biggiogera

Verified customer

提交于 Aug 09 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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