The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Belongs to the ribosomal protein S6e family.
Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
ab63053, at 1/50 dilution, staining RPS6 in formalin-fixed, paraffin-embedded human colon carcinoma tissue by immunohistochemistry. ab63053 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.