概述

  • 产品名称Anti-RPS6抗体
    参阅全部 RPS6 一抗
  • 描述
    兔多克隆抗体to RPS6
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe
    预测可用于: a wide range of other species
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RPS6.

    (Peptide available as ab42866.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates; HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF7 (Human breast adenocarcinoma cell line) SHSY-5Y (Human neuroblastoma cell line)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab40820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa).

靶标

Anti-RPS6 antibody 图像

  • All lanes : Anti-RPS6 antibody (ab40820) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-RPS6 antibody (ab40820) at 2 µg/ml

    Lane 1 : Whole cell lysate from mouse NIH-3T3 cell line
    Lane 2 : Whole cell lysate from mouse MEF cell line
    Lane 3 : Whole cell lysate from rat PC12 cell line

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP-conjugated pig anti rabbit Ig at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 28 kDa


    Exposure time : 5 minutes

    This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.

    Detection method: Supersignal West Dura

    See Abreview

  • IHC image of RPS6 staining in human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40820, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ICC/IF image of ab40820 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40820 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-RPS6 antibody (ab40820)参考文献

This product has been referenced in:
  • González A  et al. TORC1 promotes phosphorylation of ribosomal protein S6 via the AGC kinase Ypk3 in Saccharomyces cerevisiae. PLoS One 10:e0120250 (2015). Saccharomyces cerevisiae . Read more (PubMed: 25767889) »
  • Rallis C  et al. Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth. Biol Open 3:161-71 (2014). WB . Read more (PubMed: 24463365) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Saccharomyces cerevisiae Cell lysate - whole cell (BY4741)
Gel Running Conditions Reduced Denaturing (4-15%)
Loading amount 10 µg
Treatment 200ng/L Rapamycin for 1 hr
Specification BY4741
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Mai Sun

Verified customer

提交于 Dec 10 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Cell (S2 D.mel embryonic cell line)
Permeabilization Yes - 0.05% Triton X100
Specification S2 D.mel embryonic cell line
Fixative Paraformaldehyde
Username

Kelly Burke

Verified customer

提交于 Jul 22 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Zebrafish Tissue lysate - whole (whole larvae)
Specification whole larvae
Treatment 4% glucose, 4% fructose, 4% fructose + 100 nM Rapamycin
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Schizosaccharomyces pombe Cell lysate - whole cell (S.pombe whole cell lysate)
Loading amount 10 µg
Specification S.pombe whole cell lysate
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 05 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Leishmania Cell lysate - whole cell (L. amazonesis and L. donovani promastigotes)
Loading amount 50 µg
Specification L. amazonesis and L. donovani promastigotes
Gel Running Conditions Reduced Denaturing (15)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 11 2012

DISCOUNT CODE: 40497
Expiration date: 07/12/2012
I am very pleased to hear you would like to accept our offer and test ab40820 in Leishmania samples. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (various incl: NIH-3T3, MEF, PC12)
Loading amount 30 µg
Specification various incl: NIH-3T3, MEF, PC12
Gel Running Conditions Reduced Denaturing (12.5% SDS PAGE)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Oct 07 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"