Synthesized peptide derived from C-terminal of human RPS4Y1.
Extracts from COLO cells, HeLa cells and HUVEC cells; HUVEC cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 29 kDa.
1/500 - 1/1000.
Belongs to the ribosomal protein S4e family.
Contains 1 S4 RNA-binding domain.
Information by UniProt
40S ribosomal protein S4 antibody
40S ribosomal protein S4, Y antibody
40S ribosomal protein S4, Y isoform 1 antibody
Western blot - RPS4Y1 antibody (ab74709)
All lanes : Anti-RPS4Y1 antibody (ab74709) at 1/500 dilution Lane 1 : Extracts from COLO cells with no peptide Lane 2 : Extracts from HeLa cells with no peptide Lane 3 : Extracts from HUVEC cells with no peptide Lane 4 : Extracts from HUVEC cells with peptide at 5 µg Lysates/proteins at 5 µg per lane. Predicted band size : 29 kDa Observed band size : 29 kDa Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - RPS4Y1 antibody (ab74709)
Immunofluorescence analysis of HuvEc cells, using ab74709 at a dilution of 1/500. Left-hand panel: untreated sample; right-hand panel: with immunising peptide.
has not yet been referenced specifically in any publications.
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