概述

  • 产品名称
  • 描述
    兔多克隆抗体to RPS4Y1
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthesized peptide derived from C-terminal of human RPS4Y1.

  • 阳性对照
    • Extracts from COLO cells, HeLa cells and HUVEC cells; HUVEC cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 29 kDa.
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

靶标

  • 序列相似性
    Belongs to the ribosomal protein S4e family.
    Contains 1 S4 RNA-binding domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 40S ribosomal protein S4 antibody
    • 40S ribosomal protein S4, Y antibody
    • 40S ribosomal protein S4, Y isoform 1 antibody
    • MGC119100 antibody
    • MGC5070 antibody
    • ribosomal protein S4, Y linked 1 antibody
    • ribosomal protein S4, Y linked antibody
    • ribosomal protein S4, Y-linked 1 antibody
    • ribosomal protein S4Y antibody
    • RPS4Y antibody
    • RPS4Y1 antibody
    • RS4Y1_HUMAN antibody
    • S4 antibody
    • Y isoform 1 antibody
    see all

图片

  • All lanes : Anti-RPS4Y1 antibody (ab74709) at 1/500 dilution

    Lane 1 : Extracts from COLO cells with no peptide
    Lane 2 : Extracts from HeLa cells with no peptide
    Lane 3 : Extracts from HUVEC cells with no peptide
    Lane 4 : Extracts from HUVEC cells with peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 29 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of HuvEc cells, using ab74709 at a dilution of 1/500. Left-hand panel: untreated sample; right-hand panel: with immunising peptide.

文献

ab74709 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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