概述

  • 产品名称Anti-RPS27A抗体
    参阅全部 RPS27A 一抗
  • 描述
    兔多克隆抗体to RPS27A
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 73-122 (LRGGAKKRKK KSYTTPKKNK HKRKKVKLAV LKYYKVDENG KISRLRRECP) of Human RPS27A (NP_002945).

  • 阳性对照
    • 293T cell lysate. IF/ICC: HeLa cell line.

性能

应用

Our Abpromise guarantee covers the use of ab111598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 18 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性RPS27A (Ribosomal protein S27a) is a component of the 40S subunit of the ribosome. It is synthesized as a C-terminal extension protein (CEP) of ubiquitin. The fusion protein consists of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. The protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a.
  • 数据库链接
  • 别名
    • 40S ribosomal protein S27a antibody
    • CEP 80 antibody
    • CEP80 antibody
    • HUBCEP 80 antibody
    • HUBCEP80 antibody
    • Ribosomal protein S27a antibody
    • RPS 27A antibody
    • UBA 80 antibody
    • UBA80 antibody
    • UBCEP 1 antibody
    • UBCEP 80 antibody
    • UBCEP1 antibody
    • UBCEP80 antibody
    • Ubiquitin and ribosomal protein S27a antibody
    • Ubiquitin carboxyl extension protein 80 antibody
    • Ubiquitin CEP80 antibody
    see all

Anti-RPS27A antibody 图像

  • ab111598 stained HeLa cells. The cells were 100% metyhanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab111598 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-RPS27A antibody (ab111598) at 1 µg/ml + 293T cell lysate at 10 µg

    Predicted band size : 18 kDa
    Gel concentration: 10-20%,

Anti-RPS27A antibody (ab111598)参考文献

ab111598 has not yet been referenced specifically in any publications.

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