The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 18 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RPS27A (Ribosomal protein S27a) is a component of the 40S subunit of the ribosome. It is synthesized as a C-terminal extension protein (CEP) of ubiquitin. The fusion protein consists of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. The protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a.