Anti-RPS21抗体(ab90874)

概述

  • 产品名称Anti-RPS21抗体
    参阅全部 RPS21 一抗
  • 描述
    兔多克隆抗体to RPS21
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Pig, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide within residues: QNDAGEFVDL YVPRKCSASN RIIGAKDHAS IQMNVAEVDK VTGRFNGQFK, corresponding to N terminal amino acids 2-51 of Human RPS21 (NP_001015).

  • 阳性对照
    • Human placenta lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab90874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 9 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S21E family of ribosomal proteins. It is located in the cytoplasm. Alternative splice variants that encode different protein isoforms have been described, but their existence has not been verified. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
  • 细胞定位cytosolic small ribosomal subunit
  • 数据库链接
  • 别名
    • 1810049N11Rik antibody
    • 2410030A14Rik antibody
    • 40S ribosomal protein S21 antibody
    • 8.2 kDa differentiation factor antibody
    • MGC107620 antibody
    • OTTHUMP00000031485 antibody
    • Ribosomal protein S21 antibody
    • RP23-16P3.4 antibody
    • RP5-908M14.8 antibody
    see all

Anti-RPS21 antibody 图像

  • Anti-RPS21 antibody (ab90874) at 1 µg/ml + Human placenta lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 9 kDa
  • IHC-P image of RPS21 antibody (ab90874) on Colon sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 minutes at 22°C, before addition of the primary antibody at 1/750 dilution for 2 hours.

    See Abreview

Anti-RPS21 antibody (ab90874)参考文献

ab90874 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon)
Specification Colon
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C
Username

Dr. Aamir Ahmed

Verified customer

提交于 Oct 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"