概述

  • 产品名称Anti-RPS2抗体
    参阅全部 RPS2 一抗
  • 描述
    小鼠单克隆抗体to RPS2
  • 经测试应用适用于: WB, ICC/IF, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: APRGTGIVSA PVPKKLLMMA GIDDCYTSAR GCTATLGNFA KATFDAISKT YSYLTPDLWK ETVFTKSPYQ EFTDHLVKTH TRVSVQRTQA PAVATT, corresponding to amino acids 198-294 of Human RPS2

  • 阳性对照
    • This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa

性能

应用

Our Abpromise guarantee covers the use of ab58341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 1 - 5 µg/ml.
ELISA Use at an assay dependent concentration.

靶标

Anti-RPS2 antibody 图像

  • ICC/IF image of ab58341 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab58341 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Western blot against tagged recombinant protein immunogen using ab58341 RPS2 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa

Anti-RPS2 antibody (ab58341)参考文献

This product has been referenced in:
  • Bykhovskaya Y  et al. Phenotypic expression of maternally inherited deafness is affected by RNA modification and cytoplasmic ribosomal proteins. Mol Genet Metab 97:297-304 (2009). Read more (PubMed: 19482502) »

See 1 Publication for this product

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