The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 16 kDa.
Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
功能Required for pre-rRNA processing and maturation of 40S ribosomal subunits.
组织特异性Higher level expression is seen in the colon carcinoma tissue than normal colon tissue.
疾病相关Defects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1) [MIM:105650]. DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
序列相似性Belongs to the ribosomal protein S19e family.
细胞定位Nucleus. Located more specifically in the nucleoli.
Predicted band size : 16 kDa RPS19 antibody (ab57643) at 1ug/lane + K-562 cell lysate at 25ug/lane.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS19 antibody (ab57643)This image is courtesy of an abreview submitted by Aamir Ahmed, University College London, United Kingdom.
IHC-P image of RPS19 antibody (ab57643) on Kidney sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 minutes at 22°C, before addition of the primary antibody at 1/100 dilution for 2 hours.
Overlay histogram showing HCT116 cells stained with ab57643 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57643, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Anti-RPS19 antibody (ab57643)参考文献
This product has been referenced in:
Knight JR et al. Active regulator of SIRT1 is required for ribosome biogenesis and function. Nucleic Acids Res41:4185-97 (2013).
Read more (PubMed: 23462953) »
Shah ZH et al. A deacetylase-deficient SIRT1 variant opposes full-length SIRT1 in regulating tumor suppressor p53 and governs expression of cancer-related genes. Mol Cell Biol32:704-16 (2012).
Read more (PubMed: 22124156) »