概述

  • 产品名称
  • 描述
    兔多克隆抗体to RPS18
  • 经测试应用
    适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide selected from the center region of Human RPS18, conjugated to KLH (NP_072045.1; P62269).

  • 阳性对照
    • Mouse spleen tissue lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab91293 is purified through a Protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab91293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.

靶标

图片

  • IHC image of RPS18 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91293, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-RPS18 antibody (ab91293) at 1/100 dilution + Mouse spleen tissue lysate at 35 µg

    Predicted band size : 18 kDa

文献

This product has been referenced in:
  • Hu X  et al. Common housekeeping proteins are upregulated in colorectal adenocarcinoma and hepatocellular carcinoma, making the total protein a better "housekeeper". Oncotarget 7:66679-66688 (2016). WB . Read more (PubMed: 27556505) »
  • Tao-Cheng JH  et al. Effects of CaMKII inhibitor tatCN21 on activity-dependent redistribution of CaMKII in hippocampal neurons. Neuroscience 244:188-96 (2013). IHC (PFA fixed) ; Rat . Read more (PubMed: 23583761) »

See all 2 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293t)
Total protein in input
18 µg
Specification
293t
Immuno-precipitation step
Protein A/G
Username

Mr. Hadar Sinvani

Verified customer

提交于 Mar 13 2013

Thank you for your quick response and your continuous interest in testing our products.

DISCOUNT CODE: 41879
Expiration date: 27/03/2013
Value: £227.00
I am very pleased to hear you would like to accept our offer and test ab91...

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Thank you for your enquiry and interest.
This customer seems to be interested in testing ab91293 in a new application (IP) and in a new species (rabbit). Even though the homology of the rabbit RPS18 to human is 100%, it is not been properly tested...

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Thank you for contacting Abcam.

Since you are experiencing trouble with GAPHDand alpha-tubulin antibodies, then I would suggest that you try the mitochondrial antibody marker, ab122278, as tubulin is cytosolic but that does not seem to be wor...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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