概述

  • 产品名称
  • 描述
    兔多克隆抗体to RPS16
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human, Chinese hamster
    预测可用于: Mouse, Rat, Cow, Dog, Pig, Zebrafish
  • 免疫原

    A region within a synthetic peptide: MPSKGPLQSV QVFGRKKTAT AVAHCKRGNG LIKVNGRPLE MIEPRTLQYK, corresponding to N terminal amino acids 1-50 of Human RPS16

  • 阳性对照
    • Jurkat cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab26159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 相关性
    Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS16 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S9P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
  • 数据库链接
  • 别名
    • 40S ribosomal protein S16 antibody
    • Ribosomal protein S16 antibody
    • RPS 16 antibody
    • S16 antibody

Anti-RPS16 antibody 图像

  • Anti-RPS16 antibody (ab26159) at 1.25 µg/ml + Jurkat cell lysate

    Predicted band size : 16 kDa
  • Staining of human lung tissue with ab26159 (4-8 ug/ml). Positive labelled aleveolar cells are indicated with arrows. 400X magnification.
  • ICC/IF image of ab26159 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26159, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-RPS16 antibody (ab26159) at 2.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 16 kDa

Anti-RPS16 antibody (ab26159)参考文献

This product has been referenced in:
  • Sbarrato T  et al. A ribosome-related signature in peripheral blood CLL B cells is linked to reduced survival following treatment. Cell Death Dis 7:e2249 (2016). Read more (PubMed: 27253413) »
  • Lyabin DN & Ovchinnikov LP Selective regulation of YB-1 mRNA translation by the mTOR signaling pathway is not mediated by 4E-binding protein. Sci Rep 6:22502 (2016). WB . Read more (PubMed: 26931209) »

See all 7 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Chinese Hamster Cell lysate - whole cell (CHO DG44)
Loading amount
100000 cells
Specification
CHO DG44
Gel Running Conditions
Reduced Denaturing (12.5%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Sven Mathias

Verified customer

提交于 Aug 27 2012

Vielen Dank für Ihre Antwort.

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Leider ist die Homologie zwischen Chinese Hamster nicht ideal. Wir gehen davon aus dass ein Antikörper kreuzreagiert, wenn 85% oder mehrHomologie vorliegt. B...

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Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an. Hier bei Abcam verfahren wir so, dass wir einen kostenlosen Ersatz oder eine Gutschrift verschicken, falls ein Antikörper nicht so...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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