概述

  • 产品名称Anti-RPP40抗体
    参阅全部 RPP40 一抗
  • 描述
    小鼠多克隆抗体to RPP40
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-244 of Human RPP40 (AAH17871.1)

  • 阳性对照
    • human kidney lysate, in transfected 293T cell line, HeLa cell

性能

应用

Our Abpromise guarantee covers the use of ab89264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
  • 应用说明ICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-RPP40 antibody 图像

    • Anti-RPP40 antibody (ab89264) at 5 µg/ml + human kidney lysate at 50 µg

      Secondary
      HRP goat anti mouse IgG at 2500

      Predicted band size : 35 kDa
    • All lanes : Anti-RPP40 antibody (ab89264) at 5 µg/ml

      Lane 1 : transfected 293T cell line
      Lane 2 : non transfected 293T cell line

      Lysates/proteins at 25 µg per lane.

      Secondary
      HRP goat anti mouse IgG at 1/2500 dilution

      Predicted band size : 35 kDa
    • Immunofluorescent analysis of RPP40 expression in HeLa cells using ab89264 at 10 ug/ml

    Anti-RPP40 antibody (ab89264)参考文献

    ab89264 has not yet been referenced specifically in any publications.

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