The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 60 kDa.
Use at an assay dependent dilution.
The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation. Rpn3p is an essential, non ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome. The 26S proteasome is involved in the ATP dependent degradation of ubiquitinated proteins.