概述

  • 产品名称Anti-RPL9抗体
    参阅全部 RPL9 一抗
  • 描述
    兔多克隆抗体to RPL9
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    A region within synthetic peptide: LILEGNDIEL VSNSAALIQQ ATTVKNKDIR KFLDGIYVSE KGTVQQADE, corresponding to C terminal amino acids 144-192 of Human RPL9

  • 阳性对照
    • Jurkat cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Titre using peptide based assay 1/312500.

靶标

Anti-RPL9 antibody 图像

  • Lane 1 : Molecular weight markers
    Lane 2 : Anti-RPL9 antibody (ab50384) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 21 kDa
    Observed band size : 21 kDa

Anti-RPL9 antibody (ab50384)参考文献

ab50384 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Drosophila melanogaster Cell lysate - whole cell (S2 Drosophila cells)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 200000 cells
Specification S2 Drosophila cells
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Gerard Rimesso

Verified customer

提交于 Jul 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"