概述

  • 产品名称Anti-RPL8抗体
    参阅全部 RPL8 一抗
  • 描述
    山羊多克隆抗体to RPL8
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide:

    KAGRAYHKYKAKRNC

    , corresponding to internal sequence amino acids 181-194 of Human RPL8.

  • 阳性对照
    • IHC-P: Human brain (cortex) and spleen tissues WB: HepG2 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab116239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 28 kDa.
ELISA 1/128000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-RPL8 antibody 图像

  • Anti-RPL8 antibody (ab116239) at 0.01 µg/ml + HepG2 cell lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 28 kDa


    Exposure time : 1 hour
  • ab116239, at 2.5 µg/ml, staining RPL8 in formalin fixed, paraffin embedded Human brain (cortex) tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated anti-goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab116239, at 2.5 µg/ml, staining RPL8 in formalin fixed, paraffin embedded Human spleen tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated anti-goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Anti-RPL8 antibody (ab116239)参考文献

ab116239 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"