Mouse, Cow, Dog, Pig
A region within synthetic peptide: MAGEKVEKPD TKEKKPEAKK VDAGGKVKKG NLKAKKPKKG KPHCSRNPVL, corresponding to N terminal amino acids 1-50 of Human RPL6
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 2.5 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
Anti-RPL6 antibody 图像
Western blot - RPL6 antibody (ab50907)
Lane 1 :
MarkerLane 2 :
Anti-RPL6 antibody (ab50907) at 2.5 µg/mlLane 1 :
As aboveLane 2 :
Jurkat cell lysate at 10 µgSecondaryLane 2 :
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
33 kDaObserved band size :
~38 kDa (why is the actual band size different from the predicted?
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