概述

  • 产品名称
  • 描述
    兔多克隆抗体to RPL39
  • 特异性
    ab74740 detects endogenous levels of total RPL39 protein.
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human RPL39.

  • 阳性对照
    • WB: HepG2 and COLO cell extracts ICC/IF: A549 cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab74740 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 7 kDa.
ELISA 1/60000.
ICC/IF 1/500 - 1/1000.

靶标

  • 相关性
    RPL39 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the S39E family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein is the smallest, and one of the most basic, proteins of the ribosome. This gene is cotranscribed with the U69 small nucleolar RNA gene, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
  • 数据库链接
  • 别名
    • 2810465O16Rik antibody
    • 60S ribosomal protein L39 antibody
    • OTTHUMP00000023908 antibody
    • OTTMUSP00000018582 antibody
    • Ribosomal protein L39 antibody
    • RP23-43O20.1 antibody
    see all

图片

  • All lanes : Anti-RPL39 antibody (ab74740) at 1/500 dilution

    Lane 1 : HepG2 cell extracts
    Lane 2 : COLO cell extracts
    Lane 3 : COLO cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 7 kDa
    Observed band size : 7 kDa
  • ab74740 at 1/500 dilution staining RPL39 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

文献

This product has been referenced in:
  • Dave B  et al. Targeting RPL39 and MLF2 reduces tumor initiation and metastasis in breast cancer by inhibiting nitric oxide synthase signaling. Proc Natl Acad Sci U S A 111:8838-43 (2014). Human . Read more (PubMed: 24876273) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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