概述

  • 产品名称Anti-RPL36A抗体
    参阅全部 RPL36A 一抗
  • 描述
    小鼠单克隆抗体to RPL36A
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Pig, Xenopus laevis, Zebrafish, Baboon, Orangutan
  • 免疫原

    Recombinant fragment: TRRTFCKKCG KHQPHKVTQY KKGKDSLYAQ GKRRYDRKQS GYGGQTKPIF RKKAKTTKKI VLRLECVEPN CRSKRMLAIK RCKHFELGGD KKRKGQVIQF, corresponding to amino acids 7-106 of Human RPL36A (NP_066357) with a tag of 26 KDa.

  • 阳性对照
    • PC-12, NIH/3T3, HeLa and Raw 264.7 lysates. Recombinant tagged human RPL36A fragment.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 12 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged RPL36A is approximately 0.1ng/ml when used as a capture antibody.

靶标

Anti-RPL36A antibody 图像

  • Anti-RPL36A antibody (ab77733) at 1 µg/ml + Recombinant tagged human RPL36A fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 12 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-RPL36A antibody (ab77733) at 1 µg/ml + HeLa lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 12 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
  • Anti-RPL36A antibody (ab77733) at 1 µg/ml + PC-12 lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 12 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
  • Anti-RPL36A antibody (ab77733) at 1 µg/ml + Raw 264.7 lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 12 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • Anti-RPL36A antibody (ab77733) at 1 µg/ml + NIH/3T3 lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 12 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
  • Detection limit for recombinant tagged RPL36A is approximately 0.1ng/ml using ab77733 as a capture antibody at 1ug/well.

Anti-RPL36A antibody (ab77733)参考文献

ab77733 has not yet been referenced specifically in any publications.

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