Recombinant protein corresponding to amino acids 2-122 of Human RPL35 (P42766).
Human pancreas tissue, Human cell line U-2 OS
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250. Predicted molecular weight: 15 kDa.
Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
Anti-RPL35 antibody 图像
Western blot - Anti-RPL35 antibody (ab121244)
Anti-RPL35 antibody (ab121244) + MCF7 cell lysate
Predicted band size : 15 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL35 antibody (ab121244)
Immunohistochemical staining of paraffin embedded Human pancreas, using 1/25 ab121244, shows strong cytoplasmic positivity in exocrine glandular cells.
Immunocytochemistry/ Immunofluorescence - Anti-RPL35 antibody (ab121244)
Immunofluorescent staining with 4 µg/ml ab121244 of Human cell line U-2 OS shows positivity in cytoplasm.
Antibody staining is shown in green.
Anti-RPL35 antibody (ab121244)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"