概述

  • 产品名称Anti-RPL35抗体
    参阅全部 RPL35 一抗
  • 描述
    兔多克隆抗体to RPL35
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant protein corresponding to amino acids 2-122 of Human RPL35 (P42766).

  • 阳性对照
    • Human pancreas tissue, Human cell line U-2 OS

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab121244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/250. Predicted molecular weight: 15 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

靶标

Anti-RPL35 antibody 图像

  • Anti-RPL35 antibody (ab121244) + MCF7 cell lysate

    Predicted band size : 15 kDa
  • Immunohistochemical staining of paraffin embedded Human pancreas, using 1/25 ab121244, shows strong cytoplasmic positivity in exocrine glandular cells.
  • Immunofluorescent staining with 4 µg/ml ab121244 of Human cell line U-2 OS shows positivity in cytoplasm. Antibody staining is shown in green.

Anti-RPL35 antibody (ab121244)参考文献

ab121244 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab121244.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"