概述

  • 产品名称Anti-RPL30抗体
    参阅全部 RPL30 一抗
  • 描述
    小鼠单克隆抗体to RPL30
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human RPL30 protein (AAH32700) with a 26kDa tag.

  • 阳性对照
    • RPL30 transfected 293T cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 13 kDa.
ELISA Use at an assay dependent dilution. Detection limit is approximately 1ng/ml as a capture antibody when used against the immunogen.

靶标

Anti-RPL30 antibody 图像

  • Anti-RPL30 antibody (ab77328) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 13 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-RPL30 antibody (ab77328) at 5 µg/ml

    Lane 1 : RPL30 transfected 293T cell lysate
    Lane 2 : Non transfected 29T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 13 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
  • Detection limit for recombinant tagged RPL30 is approximately 1ng/ml using ab77328 as a capture antibody.

Anti-RPL30 antibody (ab77328)参考文献

ab77328 has not yet been referenced specifically in any publications.

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