概述

  • 产品名称Anti-RPL27A抗体
    参阅全部 RPL27A 一抗
  • 描述
    兔多克隆抗体to RPL27A
  • 特异性ab74731 detects endogenous levels of total RPL27A protein.
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    synthesized peptide derived from C-terminal region of human RPL27A.

  • 阳性对照
    • extracts from COLO cells and A549 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 17 kDa.
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.

靶标

Anti-RPL27A antibody 图像

  • All lanes : Anti-RPL27A antibody (ab74731) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from COLO cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 17 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of A549 cells, using ab74731 at 1/500 dilution with (+) or without (-) immunising peptide.

Anti-RPL27A antibody (ab74731)参考文献

This product has been referenced in:
  • Chandra S  et al. Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation. J Cell Sci 126:3782-95 (2013). Read more (PubMed: 23750012) »
  • Fromm-Dornieden C  et al. Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells. BMC Mol Biol 13:9 (2012). WB . Read more (PubMed: 22436005) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Mouse Cell (Mouse embryonic fibroblasts)
Specification Mouse embryonic fibroblasts
Permeabilization Yes - 0.5% Triton
Fixative Paraformaldehyde
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提交于 Aug 07 2013

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Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (gradient)
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification Mouse embryonic fibroblasts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 22°C
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提交于 Jun 26 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (gradient)
Sample Human Cell lysate - whole cell (U2OS cells)
Specification U2OS cells
Treatment Time course of serum stimulation
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Jun 13 2013

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Application Western blot
Sample Human Cell lysate - whole cell (normal and tumor colon cells)
Loading amount 30 µg
Specification normal and tumor colon cells
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Sep 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"