存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液 Preservative: 0.09% Sodium Azide
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纯度 Protein G purified
纯化说明 ab71341 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).
功能 This protein binds to a specific region on the 26S rRNA.
序列相似性 Belongs to the ribosomal protein L23P family.
翻译后修饰 N-terminus is methylated by METTL11A/NTM1.
Information by UniProt
60S ribosomal protein L23A antibody
Anti-RPL23A antibody - Carboxyterminal end 图像
Western blot - RPL23A antibody - Carboxyterminal end (ab71341)
All lanes : Anti-RPL23A antibody - Carboxyterminal end (ab71341) at 1/60 dilution Lane 1 : CEM cell line lysate Lane 2 : Hela cell line lysate Lane 3 : HepG2 cell line lysate Lysates/proteins at 35 µg per lane. Predicted band size : 18 kDa Observed band size : 20 kDa ( why is the actual band size different from the predicted?) Additional bands at : 25 kDa,50 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Anti-RPL23A antibody - Carboxyterminal end (ab71341)参考文献
This product has been referenced in:
et al. Nucleolar GTP-binding Protein-1 (NGP-1) Promotes G1 to S Phase Transition by Activating Cyclin-dependent Kinase Inhibitor p21 Cip1/Waf1. J Biol Chem 290:21536-52 (2015).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"