Anti-RPL22抗体(ab77720)

概述

  • 产品名称Anti-RPL22抗体
    参阅全部 RPL22 一抗
  • 描述
    兔多克隆抗体to RPL22
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Pig, Zebrafish, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human RPL22.

    (Peptide available as ab87822.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HeLa; HEK293; Jurkat.

性能

应用

Our Abpromise guarantee covers the use of ab77720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).

靶标

Anti-RPL22 antibody 图像

  • All lanes : Anti-RPL22 antibody (ab77720) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
    Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.

Anti-RPL22 antibody (ab77720)参考文献

ab77720 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Chicken Cell lysate - whole cell (T CELLS)
Gel Running Conditions Non-reduced Denaturing (12 %)
Loading amount 1e+006 cells
Specification T CELLS
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Ahmed kheimar

Verified customer

提交于 Jun 28 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Forebrain)
Loading amount 10 µg
Specification Forebrain
Gel Running Conditions Non-reduced Denaturing
Blocking step Odyssey blocking buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 14 2013

I am sorry that you did not recieve my original response to your question. I emailed you a reply on the 24th January 2012. Please find a copy of the email I sent below:
There are no references associated with the antibody, however we do have a wes...

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Thank you for contacting Abcam.
There are no references associated with the antibody, however we do have a western blot image for this antibody on our webpage. Under our Abpromise we do guarantee that this antibody will work in western blot on hum...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"