Anti-RPL19抗体(ab58328)

概述

  • 产品名称Anti-RPL19抗体
    参阅全部 RPL19 一抗
  • 描述
    小鼠单克隆抗体to RPL19
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARMP EKVTWMRRMR , corresponding to amino acids 1-101 of Human RPL19

  • 阳性对照
    • ICC/IF: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab58328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 23 kDa.
IHC-P Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

靶标

Anti-RPL19 antibody 图像

  • ICC/IF image of ab58328 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58328, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • RPL19 antibody (ab58328) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human small Intestine tissue.


  • Predicted band size : 23 kDa
    RPL19 antibody (ab58328) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab58328 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58328, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-RPL19 antibody (ab58328)参考文献

ab58328 has not yet been referenced specifically in any publications.

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I am very pleased to hear you would like to test ab57637 and ab58328 in Xenopus. I have performed the sequence alignment of the immunogen used to raise these antibodies to that of the most similar Xenopus sequence. Ab57637 and ab58328 matched by 97 and...

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