概述

  • 产品名称Anti-RPL11抗体
    参阅全部 RPL11 一抗
  • 描述
    兔多克隆抗体to RPL11
  • 经测试应用适用于: IHC-P, WB, ICC/IF, IPmore details
  • 种属反应性
    与反应: Human, Zebrafish
    预测可用于: Mouse, Rat, Cow, Cat, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human RPL11.

    (Peptide available as ab88221.)

  • 阳性对照
    • This antibody gave a positive signal in human liver and human brain tissue lysates and in the following whole cell lysates: HepG2; HeLa; Jurkat; Caco 2; HEK293. It also gave a positive control in human pancreas tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab79352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 21046615
IP Use at an assay dependent concentration.

靶标

  • 功能Binds to 5S ribosomal RNA (By similarity). Required for rRNA maturation and formation of the 60S ribosomal subunits. Promotes nucleolar location of PML.
  • 疾病相关Defects in RPL11 are the cause of Diamond-Blackfan anemia type 7 (DBA7) [MIM:612562]. DBA7 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
  • 序列相似性Belongs to the ribosomal protein L5P family.
  • 细胞定位Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 60S ribosomal protein L11 antibody
    • Cell growth inhibiting protein 34 antibody
    • Cell growth inhibiting protein34 antibody
    • CLL associated antigen KW 12 antibody
    • CLL associated antigen KW12 antibody
    • CLL-associated antigen KW-12 antibody
    • DBA 7 antibody
    • DBA7 antibody
    • GIG 34 antibody
    • GIG34 antibody
    • L11 antibody
    • Ribosomal protein L11 antibody
    • RL11_HUMAN antibody
    • RPL 11 antibody
    • Rpl11 antibody
    see all

Anti-RPL11 antibody 图像

  • All lanes : Anti-RPL11 antibody (ab79352) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 7 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 30 kDa,45 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • RPL11 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to RPL11 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79352.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 22kDa: RPL11
  • IHC image of ab79352 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79352, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-RPL11 antibody (ab79352) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 20 µg
    Lane 4 : Zebrafish liver homogenate at 20 µg
    Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg
    Lane 6 : Human hepatocellular carcinoma cell line at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 22 kDa


    Exposure time : 3 minutes

Anti-RPL11 antibody (ab79352)参考文献

This product has been referenced in:
  • Yoon JC  et al. GLTSCR2/PICT1 links mitochondrial stress and Myc signaling. Proc Natl Acad Sci U S A 111:3781-6 (2014). Read more (PubMed: 24556985) »
  • Havel JJ  et al. Nuclear PRAS40 couples the Akt/mTORC1 signaling axis to the RPL11-HDM2-p53 nucleolar stress response pathway. Oncogene N/A:N/A (2014). Read more (PubMed: 24704832) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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提交于 Dec 23 2013


Expiration date: 27-Mar-13


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