Anti-RPE65抗体(ab67042)

概述

  • 产品名称Anti-RPE65抗体
    参阅全部 RPE65 一抗
  • 描述
    兔多克隆抗体to RPE65
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Chicken, Cow, Human, Xenopus laevis, Non Human Primates, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human RPE65.

    (Peptide available as ab99880.)

  • 阳性对照
    • This antibody gave a positive signal in the Mouse Retina Tissue Lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab67042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa).

靶标

  • 功能Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.
  • 组织特异性Retinal pigment epithelium specific.
  • 疾病相关Defects in RPE65 are the cause of Leber congenital amaurosis type 2 (LCA2) [MIM:204100]. LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children.
    Defects in RPE65 are the cause of retinitis pigmentosa type 20 (RP20) [MIM:613794]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP20 inheritance is autosomal dominant.
  • 序列相似性Belongs to the carotenoid oxygenase family.
  • 翻译后修饰Palmitoylation by LRAT regulates ligand binding specificity; the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A).
  • 细胞定位Cytoplasm. Cell membrane. Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • All-trans-retinyl-palmitate hydrolase antibody
    • LCA 2 antibody
    • LCA2 antibody
    • Leber congenital amaurosis antibody
    • mRPE 65 antibody
    • mRPE65 antibody
    • p63 antibody
    • rd 12 antibody
    • rd12 antibody
    • Retinal pigment epithelium specific 61 kDa protein antibody
    • Retinal pigment epithelium specific 65 kDa protein antibody
    • Retinal pigment epithelium specific protein antibody
    • Retinal pigment epithelium specific protein 65kDa antibody
    • Retinal pigment epithelium-specific 65 kDa protein antibody
    • Retinitis pigmentosa 20 antibody
    • Retinoid isomerohydrolase antibody
    • Retinol isomerase antibody
    • RP 20 antibody
    • RP20 antibody
    • RPE 65 antibody
    • RPE65 antibody
    • RPE65_HUMAN antibody
    • sRPE 65 antibody
    • sRPE65 antibody
    see all

Anti-RPE65 antibody 图像

  • Anti-RPE65 antibody (ab67042) at 1 µg/ml + Mouse Retina Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes
  • All lanes : Anti-RPE65 antibody (ab67042) at 1/1000 dilution

    Lane 1 : Lepomis macrochirus (blue gill)RPE Lysate at 10 µl
    Lane 2 : Lepomis macrochirus (blue gill)RPE Lysate at 5 µl

    Secondary
    HRP congugated Goat anti-rabbit at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-RPE65 antibody (ab67042)参考文献

ab67042 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Zebrafish Tissue lysate - whole (Retina extracted w/ M-PER)
Gel Running Conditions Reduced Denaturing (10%, hand-cast)
Loading amount 35 µg
Specification Retina extracted w/ M-PER
Blocking step Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Jun 03 2016

Application Western blot
Sample Fish Cell lysate - whole cell (retinal pigment epithelium)
Loading amount 20 µg
Specification retinal pigment epithelium
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 12 2013

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