概述

  • 产品名称Anti-RPC62抗体
    参阅全部 RPC62 一抗
  • 描述
    兔多克隆抗体to RPC62
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 484-534 of Human RPC62 (NP_006459.3)

  • 阳性对照
    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab112057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 61 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
  • 序列相似性Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA directed III 62 kDa polypeptide antibody
    • DNA-directed RNA polymerase III subunit C antibody
    • DNA-directed RNA polymerase III subunit rpc3 antibody
    • POLR3C antibody
    • Polymerase (RNA) III (DNA directed) polypeptide C (62kD) antibody
    • RNA polymerase C subunit 3 antibody
    • RNA polymerase III 62 kDa subunit antibody
    • RNA polymerase III C62 subunit antibody
    • RNA polymerase III subunit C3 antibody
    • RPC3 antibody
    • RPC3_HUMAN antibody
    • RPC62 antibody
    see all

Anti-RPC62 antibody 图像

  • All lanes : Anti-RPC62 antibody (ab112057) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 61 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation of RPC62. 1 mg of HeLa Whole cell lysate IP in which 20% of IP was loaded. Blotted with rabbit anti-RPC62 antibody (ab112057) at 0.4 µg/ml. Lane 1- RPC62 was immunoprecipitated by rabbit anti- RPC62 antibody (ab112057) at 6 mcg/mg lysate; Lane 2- RPC62 was immunoprecipitated by an rabbit anti- RPC62 antibody, that recognizes an upstream epitope. Lane 3- Rabbit IgG control. Detection: Chemiluminescence with exposure times of 3 minutes.

Anti-RPC62 antibody (ab112057)参考文献

ab112057 has not yet been referenced specifically in any publications.

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