概述

  • 产品名称Anti-RPB2抗体
    参阅全部 RPB2 一抗
  • 描述
    兔多克隆抗体to RPB2
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Dog, Zebrafish
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 6 and 142 of Human RPB2 (NP_000929).

  • 阳性对照
    • A431, H1299, HeLa or HepG2 cell lines.

性能

应用

Our Abpromise guarantee covers the use of ab96797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 134 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 相关性This 140 kDa protein is the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. It is suggested that Rpb2 plays a role in enhancing transcription from prostate specific antigen genes. These genes may be linked to prostate cancer progression.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • DNA directed RNA polymerase II 140 kDa polypeptide antibody
    • DNA-directed RNA polymerase II subunit B antibody
    • hRPB140 antibody
    • hsRPB2 antibody
    • POL2RB antibody
    • Polymerase (RNA) II (DNA directed) polypeptide B (140kD) antibody
    • RNA polymerase II 140kD subunit antibody
    • RNA polymerase II second largest subunit antibody
    • RNA polymerase II subunit 2 antibody
    • RNA polymerase II subunit B2 antibody
    see all

Anti-RPB2 antibody 图像

  • Anti-RPB2 antibody (ab96797) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 134 kDa
  • Top: immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using ab96797 at 1/200 dilution.
    Bottom: image merged with DNA probe.
  • Immunohistochemistry analysis of paraffin-embedded NCIN87 xenograft using ab96797 at 1/500 dilution.

Anti-RPB2 antibody (ab96797)参考文献

ab96797 has not yet been referenced specifically in any publications.

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