Mouse, Cow, Dog, Zebrafish
Recombinant protein fragment corresponding to a region within amino acids 6 and 142 of Human RPB2 (NP_000929).
A431, H1299, HeLa or HepG2 cell lines.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 40% Glycerol, PBS, pH 7
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500.
1/500 - 1/3000. Predicted molecular weight: 134 kDa.
1/100 - 1/200.
This 140 kDa protein is the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. It is suggested that Rpb2 plays a role in enhancing transcription from prostate specific antigen genes. These genes may be linked to prostate cancer progression.
DNA directed RNA polymerase II 140 kDa polypeptide antibody
DNA-directed RNA polymerase II subunit B antibody
Western blot - RPB2 antibody (ab96797)
Anti-RPB2 antibody (ab96797) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size : 134 kDa
Immunocytochemistry/ Immunofluorescence - RPB2 antibody (ab96797)
Top: immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using ab96797 at 1/200 dilution.
Bottom: image merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RPB2 antibody (ab96797)
Immunohistochemistry analysis of paraffin-embedded NCIN87 xenograft using ab96797 at 1/500 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"