概述

  • 产品名称Anti-RPB2抗体
    参阅全部 RPB2 一抗
  • 描述
    兔多克隆抗体to RPB2
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Xenopus tropicalis
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human DNA directed RNA polymerase II 140 kDa subunit encoded within exon 4 (LocusLink ID 5431).

  • 阳性对照
    • Whole cell lysate from 293T cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab10332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 134 kDa).
IP Use a concentration of 2 - 5 µg/ml.

靶标

  • 相关性This 140 kDa protein is the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. It is suggested that Rpb2 plays a role in enhancing transcription from prostate specific antigen genes. These genes may be linked to prostate cancer progression.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • DNA directed RNA polymerase II 140 kDa polypeptide antibody
    • DNA-directed RNA polymerase II subunit B antibody
    • hRPB140 antibody
    • hsRPB2 antibody
    • POL2RB antibody
    • Polymerase (RNA) II (DNA directed) polypeptide B (140kD) antibody
    • RNA polymerase II 140kD subunit antibody
    • RNA polymerase II second largest subunit antibody
    • RNA polymerase II subunit 2 antibody
    • RNA polymerase II subunit B2 antibody
    see all

Anti-RPB2 antibody 图像



  • Predicted band size : 134 kDa


    Detection of RPB2 using ab10332 by Western blot. Samples: Whole cell lysate (50 mg) from 293 cells. From left to right: ab10332 concentrations 1, 0.5, 0.5 and 2mg/ml; two left hand columns after 1 minute’s exposure and two right hand columns after 2 hours’ exposure. Band representing RPB2 at approximately 140kDa.
  • Detection of RPB2 using ab10332 by immunoprecipitation. Samples: Whole cell lysate from 293 cells. Antibodies: ab10332 used at 10mg/10cm IP plate. Detection: chemiluminescence with exposure time of 10 seconds.

Anti-RPB2 antibody (ab10332)参考文献

This product has been referenced in:
  • Brem R  et al. Reactive oxygen species generated by thiopurine/UVA cause irreparable transcription-blocking DNA lesions. Nucleic Acids Res 37:1951-61 (2009). WB ; Human . Read more (PubMed: 19208641) »

See 1 Publication for this product

Product Wall

The immunogen for this antibody is a peptide between residues 75 and 125 of human RNA Polymerase 2 Polypeptide B using the numbering given in entry NP_000929.1. That is, it is a peptide contained within the following range:

AQHASGEVEEPPRYLLK...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"