Anti-RPA70抗体(ab87272)
Key features and details
- Rabbit polyclonal to RPA70
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-RPA70抗体
参阅全部 RPA70 一抗 -
描述
兔多克隆抗体to RPA70 -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan -
免疫原
Synthetic peptide, corresponding to a region within amino acids 350 - 400 of Human RPA70 (NP_002936.1)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab87272于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. Antigen retrieval with tris-EDTA buffer pH 9.0 recommended. Likely to work with frozen sections.
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说明 |
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IHC-P
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. Antigen retrieval with tris-EDTA buffer pH 9.0 recommended. Likely to work with frozen sections. |
靶标
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功能
Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
序列相似性
Belongs to the replication factor A protein 1 family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison. Desumoylated by SENP6. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6117 Human
- Entrez Gene: 68275 Mouse
- Omim: 179835 Human
- SwissProt: P27694 Human
- SwissProt: Q8VEE4 Mouse
- Unigene: 461925 Human
- Unigene: 180734 Mouse
- Unigene: 472099 Mouse
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别名
- Dmrpa1 antibody
- Drosophila Replication Protein A antibody
- DRPA antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab87272 被引用在 8 文献中.
- Takemoto K et al. Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic Recombination. Cell Rep 31:107686 (2020). WB ; Mouse . PubMed: 32460033
- Xu Y et al. A cell-based high-content screen identifies isocotoin as a small molecule inhibitor of the meiosis-specific MEIOB-SPATA22 complex†. Biol Reprod N/A:N/A (2020). PubMed: 32463099
- Qin Y et al. WDR62 is involved in spindle assembly by interacting with CEP170 in spermatogenesis. Development 146:N/A (2019). PubMed: 31533924
- Hua R et al. FBXO47 regulates telomere-inner nuclear envelope integration by stabilizing TRF2 during meiosis. Nucleic Acids Res 47:11755-11770 (2019). PubMed: 31724724
- Tian H et al. CXXC1 is not essential for normal DNA double-strand break formation and meiotic recombination in mouse. PLoS Genet 14:e1007657 (2018). PubMed: 30365547
- Xu Z et al. H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation. Nucleic Acids Res 44:9681-9697 (2016). PubMed: 27431324
- Luo M et al. MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination. Nat Commun 4:2788 (2013). PubMed: 24240703
- Shin YH et al. Hormad1 mutation disrupts synaptonemal complex formation, recombination, and chromosome segregation in mammalian meiosis. PLoS Genet 6:e1001190 (2010). ICC/IF ; Mouse . PubMed: 21079677