概述

  • 产品名称Anti-RPA70抗体
    参阅全部 RPA70 一抗
  • 描述
    兔多克隆抗体to RPA70
  • 经测试应用适用于: IP, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human replication protein A1, 70kDa encoded within exon 12 (LocusLink ID 6117).

  • 阳性对照
    • Hela cells.

性能

应用

Our Abpromise guarantee covers the use of ab12320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 3-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000 - 1/25000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).

靶标

  • 功能Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • 序列相似性Belongs to the replication factor A protein 1 family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison. Desumoylated by SENP6.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dmrpa1 antibody
    • Drosophila Replication Protein A antibody
    • DRPA antibody
    • HSSB antibody
    • Human single stranded DNA binding protein antibody
    • MST075 antibody
    • MSTP075 antibody
    • p70 antibody
    • REPA1 antibody
    • Replication factor A antibody
    • Replication factor A protein 1 antibody
    • Replication protein A 70 kDa DNA-binding subunit antibody
    • Replication protein A 70kDa DNA binding subunit antibody
    • Replication protein A1 70kDa antibody
    • Replication protein A1 antibody
    • RF A antibody
    • RF-A protein 1 antibody
    • RFA antibody
    • RFA1_HUMAN antibody
    • RP A antibody
    • RP-A p70 antibody
    • RPA 70 antibody
    • RPA antibody
    • rpa1 antibody
    • Single stranded binding protein 70 antibody
    • Single-stranded DNA-binding protein antibody
    see all

Anti-RPA70 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling RPA70 with ab12320 at 1/1000 (1 µg/ml). Detection: DAB.


  • Predicted band size : 74 kDa
    Detection of Human RPA70 by Western Blot. Samples: Whole cell lysate (10, 50 and 100 mcg/lane in lanes 1, 2 and 3, respectively) from HeLa cells. Antibody: ab12320 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.

     

    Detection of Human RPA70 by Western Blot.
    Samples: Whole cell lysate (10, 50 and 100 mcg/lane in lanes 1, 2 and 3, respectively) from HeLa cells.
    Antibody: ab12320 used at the indicated concentrations.
    Detection: Chemiluminescence with a 10 second exposure.

Anti-RPA70 antibody (ab12320)参考文献

This product has been referenced in:
  • Scarzello AJ  et al. A Mutation in the SH2 domain of STAT2 prolongs tyrosine phosphorylation of STAT1 and promotes type I IFN-induced apoptosis. Mol Biol Cell 18:2455-62 (2007). WB ; Human . Read more (PubMed: 17442890) »
  • McCabe N  et al. Deficiency in the repair of DNA damage by homologous recombination and sensitivity to poly(ADP-ribose) polymerase inhibition. Cancer Res 66:8109-15 (2006). Read more (PubMed: 16912188) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry. My colleague, who was initially dealing with your enquiry is currently out of the office. The immunogen used to raise this product was a peptide sequence within the region of amino acid 350 and 400 of the human replication p...

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Thank you for your enquiry. I have details of the sequence alignment between the N. crassa RPA1 and the epitope of the RPA70 antibody (ab12320). Unfortunately the epitope is not conserved between human and N. crassa RPA1 and therefore the antibody ...

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Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should y...

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We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"